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基质金属蛋白酶(MMP2/9)明胶酶谱法试剂盒

基质金属蛋白酶(MMP2/9)明胶酶谱法试剂盒

CHANPINSHIJIAN:2015-11-06

简要描述:

基质金属蛋白酶(matrix metalloproteinase, MMP)以无活性的酶原形式分泌,活化后能够降解细胞外基质的胶原蛋白,又称胶原酶。血浆与组织中的MMP-2、MMP-9参与各种生理与病理过程,其在临床诊断和治疗中的意义日益受到重视。本试剂盒可进行50 次标准小胶检测,如果小胶加样孔为10~15
个,则总计可检测500~750 个样品。基质金属蛋白酶(MMP2/9)明胶酶谱法试剂盒

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NANJINGXINFANSHENGWUJISHUYOUXIANGONGSIGONGYINGGONGYINGMMP-2 ,MMP-9MINGJIAOMEIPUFASHIJIHE,MINGJIAOMEIPUFASHIJIHECUXIAO,MMP-2SHIJIHE。

基质金属蛋白酶(MMP2/9)明胶酶谱法试剂盒

MMP Zymography Assay Kit (for MMP-2 and MMP-9) 

MIAOSHU:JIZHIJINSHUDANBAIMEI(matrix metalloproteinase, MMP)YIWUHUOXINGDEMEIYUANXINGSHIFENMI,HUOHUAHOUNENGGOUJIANGJIE

XIBAOWAIJIZHIDEJIAOYUANDANBAI,YOUCHENGJIAOYUANMEI。TONGGUOYINGXIANGXIBAOWAIJIZHI,JIAOYUANMEICANYUPEITAIFAYU、XINGTAIFASHENG、ZU

ZHIZHONGSUDENGGUOCHENG,BINGCANYUYANZHENG、ZHONGLIU、XINXUEGUAN、SHENJINGDENGXUDUOJIBINGGUOCHENG。XUEJIANGYUZUZHIZHONGDEMMP-2、MMP-9

上海11选5CANYUGEZHONGSHENGLIYUBINGLIGUOCHENG,QIZAILINCHUANGZHENDUANHEZHILIAOZHONGDEYIYIRIYISHOUDAOZHONGSHI1。

上海11选5BENSHIJIHECAIYONGMEIPUFA(zymography)JIANCEMMP-2、MMP-9 HUOXING。Zymography SHIYIZHONGGUANGWEISHIYONGDE、JI

YUSDS-PAGE DIANYONGHEFANXIANGNINGJIAORANSEDEDANBAIMEIJIANCEFANGFA,QILINGMINDUKEDA1 nM,GAOYUELISA FANGFA2,QI

JIBENYUANLIHECHENGXUSHI:ZHIBEIJIAYOUJIAOYUANMEIDIWUDESDS-PAGE NINGJIAO,HANDANBAIMEIDEYANGPINZAICININGJIAOZHONGJINXINGDIAN

上海11选5YONG, DIANYONGJIESHUHOUQUCHUNINGJIAOYUMEIFANYINGBuffer FUYU,NINGJIAORANSEYUTUOSE。NINGJIAOZHONGYOUYUHANDIWUDANBAIERBEISHEN

RAN,XINGCHENGSHENSEBEIJING,DANZAIYOUDANBAIMEITIAODAIDEWEIZHI,DANBAIMEIJIANGJIANGJIENINGJIAOZHONGDEDIWUDANBAI,YINERBUBEIRANSE

上海11选5ERXINGCHENGTOULIANGQUYU,CONGERNENGTONGSHIZHISHIMMP-2、MMP-9 DEDAXIAOWEIZHI(MEIPU)JIHUOXING。BENSHIJIHETIGONG

MMP-2、MMP-9 TEYIDANBAIDIWUJIMEIXUEFANYINGHUANCHONGYE,KEJIANCESHAOZHI0.1~0.5 μl XUEYEZHONGDEMMP-2、MMP-

上海11选59 MEIPUJIHUOXING,JIANCELINGMINDU~1 nM。SHIJIHEKEJINXING50 CIBIAOZHUNXIAOJIAOJIANCE,RUGUOXIAOJIAOJIAYANGKONGWEI10~15

上海11选5GE,ZEZONGJIKEJIANCE500~750 GEYANGPIN。

基质金属蛋白酶(MMP2/9)明胶酶谱法试剂盒

上海11选5适用: 检测MMP-2、MMP-9 酶谱及活性(Detect MMP2 and MMP9 as low as 1

nM)。ZUCHENGYUCHUCUN(50 assays):

上海11选51. 2 × SDS-PAGE non-reducing buffer 1.5 ml μl, ?20 oC;

2. 10 × Substrate G, 50 ml, ?20 oC;

3. 10 × Buffer A, 50 ml, 4 oC, SHIYONGSHIYONGZHENGLIUSHUIXISHI;

上海11选54. 10 × Buffer B, 50 ml, 4 oC, SHIYONGSHIYONGZHENGLIUSHUIXISHI;

上海11选55. SDS-PAGE NINGJIAODANBAIZHIKAOMASILIANGLANRANSEYE(#P1501), 4 oC。

JIANCEBUZHOU:

上海11选51. ZHIBEIHANMMP DIWUDANBAIDESDS-PAGE NINGJIAO:TUIJIANFENLIJIAONONGDUWEI8%。ANZHAOBIAOZHUNCHENGXUZHIBEISDSPAGE

NINGJIAO。JIANG10 × substrate G RONGHUA,BING90 oC JIARE5 FENZHONG。FENBIEZAINONGSUOJIAOHEFENLIJIAOZHONGEWAIJIA

上海11选5RU10 × substrate G BINGSHIZHIXISHI10 BEI,HUNYUNHOUJIARUGUOLIUSUANANHETEMED,DENGDAININGJIAOJUHE。

上海11选52. DAICEYANGPIN1:1 XISHIYU2 × SDS-PAGE non-reducing buffer (YONGWANHOUKEZIXINGPEIZHI:4% SDS, 100 mM

Tris-Cl pH6.8, 20% glycerol, 0.02% bromophnol blue),HUNHEHOUZHIJIESHANGYANG。QIEWUJIAREBIANXING,BINGQIEJIN

SHIYONGBUJIAHAIYUANJIDESHANGYANGHUANCHONGYE。KETONGGUOYUSHIYANQUEDINGJIAYANGLIANG,SHIYONGPUTONGDANBAIYURANMarker JIKE。YANG

XINGDUIZHAO(KEXUANBUZHOU):KEQUREN、XIAOSHU、DASHUHUOTU100μl QUANXUEYU100μl 2 × SDS-PAGE nonreducing

上海11选5buffer DENGTIJIHUNHE,QU5-15μl SHANGYANG。

上海11选53. DIDIANLIUHENGLIUDIANYONG,MEIYIKUAIXIAOJIAODIANLIUWEI20 mA/gel。XIUFENLANPAOCHUNINGJIAOQIANYANSHIJIESHUDIANYONG。

4. XIDININGJIAO:QUCHUNINGJIAO,QUCHUNONGSUOJIAO,FANGRURONGQINEI。KEXIANYONGSHILIANGZHENGLIUSHUICHONGXININGJIAO,RANHOUJIARU10 ml

上海11选51× Buffer A(YONGZHENGLIUSHUIXISHI),SHIWENPIAOXININGJIAO2 × 30 FENZHONG。ZHONGJIANHUANYE。

上海11选55. FUYU:DAODIAOBuffer A。JIARU10 ml 1× Buffer B(ZHENGLIUSHUIXISHI),SHIWENHUO37oC FUYU1~5 XIAOSHI。YANGXING

DUIZHAOHUOZHEXUEZHONGDEMMP TONGCHANG37oC FUYU1 XIAOSHIJIKEXIANSHI。RUMMP HUOXINGDI,YINGYANZHANGFUYUSHIJIANWEI

10 XIAOSHIHUOGUOYE。

上海11选56. XIANSE:DAODIAOBuffer B,JIARUSDS-PAGE NINGJIAODANBAIZHIKAOMASILIANGLANRANSEYE(CAOZUOBUZHOUJIANSDS-PAGE

NINGJIAODANBAIZHIKAOMASILIANGLANRANSEYESHUOMINGSHU)。NINGJIAODEDABUFENQUYUBEISHENRAN,ZAIYOUMMP TIAODAIDEWEIZHI

上海11选5BUBEIRANSEERXINGCHENGTOULIANGQUYU。TOUMINGQUYUDEWEIZHIHEFANWEIZHISHIMMP-2、MMP-9 DEDAXIAOWEIZHI(MEIPU)JI

HUOXING。YANGXINGDUIZHAOJIANGZAI66~72 (MMP-2)、92 (MMP-9)、130 (proMMP-9)、225 (proMMP-9) kDa

WEIZHICHUXIANTOUMINGTIAODAI。

7. TIAODAISAOMIAO:JIANGNINGJIAOFANGZAISAOMIAOYISHANGSAOMIAO。SUIRANMMP TIAODAITOUMING,DANNINGJIAOBEIJINGBINGFEIZHENZHENGQUANHEI。JIEJUE

BANFA:KEYONGFEITOUSHEGUANGSAOMIAO,HUOYONGRUANJIANTUXIANGCHULICHENGXUDIAOZHENGBEIJINGXIANSHIWEIHUIDUXIANSHI,BINGJINLIANGSHEZHIWEIHEI

SE。MMP TIAODAIZEWEIBAISE,ZHEIZHONGBEIJINGHEITIAODAIBAIDEGESHISHIYINGWENLUNWENZHONGzuiCHANGJIANDEGESHI。

上海11选5Page 2 of 2 DocRev: 201308

SHUOMING

上海11选51. 10 mM NENGGOUEDTA WANQUANYIZHIMMP HUOXING。

2. NINGJIAOGANZAO:KESHIYONGJUBINGXIXIANANNINGJIAOGANJIAOZHUANGZHI(#P2000)SHIWENKUAISUGANZAONINGJIAO,ZUOWEI*JILUBAOLIU。

CANKAOWENXIAN:

1. Nagase H and Woessner JF. Matrix Metalloproteinases. J Biol Chem, 1999, 274, 21491-21494

2. Heussen C, and Dowdle EB. Electrophoretic analysis of plasminogen activators in polyacrylamide

gels containing sodium dodecyl sulfate and copolymerized substrates. Anal. Biochem,1980, 102,

196–202

上海11选5SDS-PAGE NINGJIAODANBAIZHIKAOMASILIANGLANRANSEYESHUOMINGSHUP1501

CHANGGUIKAOMASILIANGLANSDS-PAGE NINGJIAODANBAIZHIRANSESHISHIYANSHICHANGYONGDENINGJIAORANSEFANGFA。YINRANFANGFASUIRANLINGMINDU

GAO,DANSUOXUSHIJIFUZABINGQIEYOUDUYOUHAI,CAOZUOBUZHOUFANSUO,NANYIKONGZHIHUODEHAODERANSEXIAOGUO。

RANSEBUZHOU:

上海11选51. CIRANSEYEJIWEIGONGZUOYE,SHIYONGSHIZHIJIEJIANGJUBINGXIXIANANNINGJIAOFANGZAIPEIYANGMINZHONGYIKAOMASILIANGLANRANSEYEFUGAINING

JIAO,BINGHUANMANYAODONG2h;

上海11选52. QINGQURANSEYE,YONGTUOSEYECHONGXININGJIAO;

3. YITUOSEYEFUGAININGJIAO,HUANMANYAODONG2h,QINGQUTUOSEYE,ZAIJIARUXINTUOSEYEJINXINGTUOSE,ZHIZHIHUODEQINGXIDE

上海11选5LANSEDETIAODAIHEGANJINGDEBEIJING(TONGCHANGCIGUOCHENGXUYAO2~4h,YEKETUOSEGUOYEZHIQINGXIDELANSEDETIAODAIHEGANJING

DEBEIJING);

上海11选54. DUININGJIAOJINXINGFENXIHEZHAOXIANG,NINGJIAOKEFANGZHIZAI7%DEYISUANZHONGBAOCUN。YEKEYONGNINGJIAOGANJIAOZHUANGZHI(P2000)DUI

NINGJIAOJINXINGCHULIHOUBAOCUN。

ANQUANXING:HANYOUJIACHUN,WUTESHUDUXING,ANYIBANHUAXUEPINCAOZUOGUICHENGCHULI。

SHUOMING:

上海11选51. RANSEYEPEIFANG:0.25g KAOMASILIANGLANR250+420ml JIACHUN+100ml BINGYISUAN,DINGRONGZHI1000ml,HUNHE1h HOUYONG

上海11选5Whatman 1 HAOLVZHIGUOLV,YUSHIWENKEWUXIANQIBAOCUN;

2. TUOSEYEPEIFANG:BINGCUSUAN:JIACHUN:SHUI=7:5:88(V:V:V);

3. BAOCUNYE:7%BINGCUSUAN;

上海11选54. NINGJIAORANSEZHIHOU,RANSEYEKEYIHUISHOULIYONG,ZHUANGRUXINRONGQINEI,SHIWENHUO4 oC BAOCUN,KEFANFUSHIYONG2-4 CI。


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